More than 220 MPs have urged Sir Keir Starmer to immediately recognise Palestine as a state.
Some 221 MPs, who span nine parties, wrote to the Prime Minister and Foreign Secretary David Lammy on Friday. The letter, organised by Sarah Champion MP who chairs the International Development Select Committee, comes ahead of an upcoming UN Conference in the United States next week.
It said: "We are expectant that the outcome of the conference will be the UK Government outlining when and how it will act on its long-standing commitment on a two-state solution; as well as how it will work with international partners to make this a reality."
Parliamentarians from Labour, the Conservatives, Lib Dems, SNP, Greens, Plaid Cymru, SDLP and independents were among those who signed the letter. Senior signatories include Labour select committee chairs including Liam Byrne and Ruth Cadbury, and Lib Dem leader Sir Ed Davey.
But a Cabinet minister defended the Government's resistance to calls for the UK to recognise a Palestinian state immediately amid mounting global anger over the starving population in Gaza.
Technology Secretary Peter Kyle insisted Prime Minister Sir Keir Starmer wants sovereignty agreed "more than anyone" but said the status must be reached as part of a political process.
It comes amid calls from both opposition critics and Labour ranks for the Government to support statehood after France became the biggest and most powerful European country to recognise Palestine.
Speaking to broadcasters, Mr Kyle said the road to sovereignty was "in the gift of Palestine and Israel" through ceasefire negotiations, adding that Britain's immediate focus was on helping to restore aid to Gaza.
"Keir Starmer wants this more than anyone else, but believes it is a crucial step towards delivering the peace and security into the future, and needs to be a negotiated peace within the region itself. It can't be forced," he told Sky News.
"We want Palestinian statehood. We desire it, and we want to make sure the circumstances can exist where that kind of long-term political solution can have the space to evolve and make sure that it can become a permanent circumstance that can bring peace to the entire region.
Downing Street has faced growing pressure over its stance on Palestinian statehood since French President Emmanuel Macron made his country the first G7 nation to recognise sovereignty.
He said he would formalise the move at the UN General Assembly in September.
"But right now, today, we've got to focus on what will ease the suffering, and it is extreme, unwarranted suffering in Gaza that has to be the priority for us today."
On Friday evening, the Prime Minister said: "The appalling scenes in Gaza are unrelenting. The continued captivity of hostages, the starvation and denial of humanitarian aid to the Palestinian people, the increasing violence from extremist settler groups, and Israel's disproportionate military escalation in Gaza are all indefensible.
"Alongside our closest allies, I am working on a pathway to peace in the region, focused on the practical solutions that will make a real difference to the lives of those that are suffering in this war.
"That pathway will set out the concrete steps needed to turn the ceasefire so desperately needed, into a lasting peace.
"Recognition of a Palestinian state has to be one of those steps. I am unequivocal about that. But it must be part of a wider plan which ultimately results in a two-state solution and lasting security for Palestinians and Israelis. This is the way to ensure it is a tool of maximum utility to improve the lives of those who are suffering - which of course, will always be our ultimate goal."
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